ISSN 2321–3647
Sun, 19 Nov 2017

Development of a RP-HPLC Method for Simultaneous Determination of Atorvastatin Calcium and Clopidogrel Bisulphate in Pharmaceutical Formulation

P.Venkateswar Rao1*, Konda Ravi Kumar2, N. Srinivasa Rao1

1. Department of Pharmaceutical Analysis, Vikas College of Pharmacy, Vissannapet, Krishna Dist, A.P

2. Department of Pharmaceutical Chemistry, Hindu college of Pharmacy, Amaravathi road, Guntur, A.P.


An isocratic reversed-phase liquid chromatograpic assay method was developed for the quantitative determination of Atorvastatin calcium (ATOR) and Clopidogrel bisulphate (CLOP) in combined dosage form. A Zodiac C18, 250x4.6mm, 5µm column and OPA Buffer: ACN (70: 30,v/v) as mobile phase. The flow rate was 1mL/min and effluents were monitored at 241 nm. The retention times of Atorvastatin and Clopidogrel were 5.8 min and 3.5 min respectively. The correlation coefficient was found to be 0.99947 (for ATOR) and 0.99944 (for CLOP). The proposed method was validated with respect to linearity, accuracy, precision, and robustness. The method was successfully applied to the estimation of Atorvastatin and Clopidogrel in combined capsule dosage form.

Keywords:RP-HPLC, Atorvastatin, Clopidogrel, Formulation, Capsule, Validation.

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