ISSN 2321–3647
Sun, 19 Nov 2017

GC-MSAnalysisof Oil Isolated and Antioxidant Activity of ShorearobustaOleoresin

Shakkeela Yusuf1*, K K Srinivasan2

1. Department of Pharmaceutical Sciences, Cheruvandoor Campus, Ettumanoor, Kottayam, Kerala, India-686631.

2. Department of Chemistry, ShriMadhwaVadiraja Institute of Technology and Management, (Affiliated toVITU,Belgavi), BantakalUdupi, Karnataka -574115.


ABSTRACT

Shorearobusta widely distributed in moist and dry forest in the tropics, has been paradoxically described as deciduous, semi-decidous or evergreen species. The resin is astringent sweet acrid, cooling anodyne, vulnerary, antibacterial, deodorant constipating and detergent. It is useful in hyperhydrosis, vitiated conditions of pitta, wound ulcers, pruritis, fractures, fever, diarrhoea, dysentery etc. The main aim of the present study was to carry out the in vitro antioxidant activity of ethanolic extract Shorearobusta oleoresin and GC-MS analysis of the oil obtained from petroleum ether fraction by column chromatography. In vitro antioxidant activity screening was done by oxygen radical scavenging assay such as DPPH and Iron chelating method and the IC50 was found to be 357.44and 151.27µg/mlusing ascorbic acidas standardwith IC50 value of 52.28 and 17.85µg/mlrespectively.Total antioxidant was 94.95µg equivalent to that of ascorbic acid.Thirty chemical constituents have been identified by GC-MS; the major chemical constituents are 7-Tetracyclo [6.2.1.0(3.8)0(3.9)]undecano (7.23%), Bis(2-ethylhexyl) phthalate (7.06%),3-Octenoic acid, methyl(tetramethylene)Si (6.25), Ursa-9(11),12-dien-3-ol (4.96%), phenol (4.68%) and 1-Fluoroforskolin (4.08%).

Keywords: Shorearobusta, GC-MS,antioxidant,radical scavenging,Column chromatography, chemical constituents.


[PDF]  Viewed: 504  Downloaded: 85
  • Call for paper
    Call for paper

    American Journal of Pharmacy and Health Research (AJPHR) is a peer-reviewed, monthly official international journal allowing access to abstracts and full-text.

  • Email & SMS Alerts
    Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics
    Article Statastics

    American Journal of Pharmacy and Health Research (AJPHR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission
    Online Submission

    American Journal of Pharmacy and Health Research (AJPHR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter