ISSN 2321–3647
Sat, 18 Nov 2017

Development and Validation of Common RP-HPLC Method for Estimation of Selected Triptans

K.Veditha1*, T.E.G.K.Murthy2

1. Vignan Pharmacy College, Vadlamudi, Guntur District, Andhra Pradesh.

2. Bapatla College of Pharmacy, Bapatla, Guntur District, Andhra Pradesh.


This paper describes the common analytical method suitable for the estimation of selected triptans (Naratriptan, Sumatriptan succinate, Zolmitriptan) by reversed phase high performance liquid chromatography (RP-HPLC). Chromatographic separations were conducted on Phenomenex Luna, C18 250 X 4.6 mm, 5 microns column at room temperature using 6.8 pH phosphate buffer: acetonitrile (60: 40) as a mobile phase at a flow rate of 1.0ml min-1, while UV detection was performed at 225nm. The retention time was found to be 3.130, 3.153, 2.143min respectively for the selected triptans. The method was found to be linear in the range of 2-10µg ml-1 for all the drugs. The proposed method is having good sensitivity due to low LOD and LOQ values. Analytical recovery was >99.3%. The method was validated statistically and applied for the quantitative analysis of triptans in bulk and formulations.

Keywords: Triptans, RP-HPLC, analytical method, validation.

[PDF]  Viewed: 617  Downloaded: 88
  • Call for paper
    Call for paper

    American Journal of Pharmacy and Health Research (AJPHR) is a peer-reviewed, monthly official international journal allowing access to abstracts and full-text.

  • Email & SMS Alerts
    Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics
    Article Statastics

    American Journal of Pharmacy and Health Research (AJPHR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission
    Online Submission

    American Journal of Pharmacy and Health Research (AJPHR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter