ISSN 2321–3647
Sun, 24 Sep 2017

The Histological Effects of Ethanolic Leaf Extract of Annona muricata on the Testes of Adult Wistar Rats

Ezejindu DN*1, Udemezue OO1, Chukwujekwu IE1, Nwajago GI1, Uchefuna RC2, Maduka SO2, Ezejindu CN3

1. Department of Anatomy, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.

2. Department of Physiology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.

3. Department of Microbiology, Abia State University, Uturu, Abia State, Nigeria


ABSTRACT

This study was carried out to evaluate the effects of Annona muricata ethanolic leaf extract on the testes of adult wistar rats. Twenty wistar rats weighing between 180 – 205g were used for the study. They were designated into four groups (A, B, C & D) of five animals each. Group A animals served as the control and were orally administered 0.3ml of distilled water; the experimental groups B, C & D were orally administered 0.2 ml, 0.4ml and 0.6ml of Annona muricata ethanolic leaf extract respectively for twenty eight days. Twenty four hours after the last administration, the animals were weighed, sacrificed under the influence of chloroform vapour and dissected. Testes tissue were removed, weighed and fixed in 10% formalin for histological studies. The final body weight of groups C and D decreased significantly (P<0.001) compared with the control. The relative organ weight of groups C and D increased significantly (P<0.001) compared with the control. The mean relative organ weight of group B was statistically similar with the control. Histological findings revealed necrotic changes in the interstitial tissue, loss of spermatide changes and multinucleated giant cells in group C and D. From these findings, Annona muricata ethanolic leaf extract administered in high doses may cause histopathological alterations/lesions in the testicular cells.

Keywords: Annona muricata, Body weight, Organ weight, Wistar rats, Spermatide.


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