ISSN 2321–3647
Sat, 18 Nov 2017

Comparison of normal and reverse phase HPLC study of meloxicam

Husnul Maab*1

1. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hajvery University, Lahore, Pakistan


ABSTRACT

In this study, work was done for the development of new precise, accurate and sensitive analytical methods for the evaluation of Meloxicam by reverse and normal phase HPLC. After development of two separate methods they are compared to determine the more authentic method of the two. In reverse phase HPLC, C18 column was used and mobile phase was acetonitrile and methanol. Similarly chloroform was used as mobile phase with silicon column for normal phase HPLC. Separately equal volume of standard solution and sample solutions in HPLC vials were injected in auto sampler compartment of HPLC. Chromatogram peak areas of Meloxicam in standard and sample solutions of different concentrations were recorded and compared. These methods are later validated in different ways. The calibration curve proved to have linearity coefficient of 0.999 for reverse and 0.994 for normal phase HPLC. The precision was equivalent to 0.0003%for reverse and 0.003%for normal phase. The LOD and LOQ were 0.0003ug/ml and 0.001ug/ml respectively for reverse and 0.002 and 0.006ug/ml for normal phase. The system also showed accuracy over the range of 95 to 99% for reverse and 91 to 97% for normal phase. These methods showed accuracy, reproducibility and sensitivity and reverse phase proved to be better than normal phase but both can be used alternatively.

Key words: Meloxicam, acetonitrile, methanol, HPLC.


[PDF]  Viewed: 953  Downloaded: 142
  • Call for paper
    Call for paper

    American Journal of Pharmacy and Health Research (AJPHR) is a peer-reviewed, monthly official international journal allowing access to abstracts and full-text.

  • Email & SMS Alerts
    Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics
    Article Statastics

    American Journal of Pharmacy and Health Research (AJPHR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission
    Online Submission

    American Journal of Pharmacy and Health Research (AJPHR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter