ISSN 2321–3647
Sat, 18 Nov 2017

Establishment of Model of Visceral Pain due to Descending Colonal Distenstion and its Behavioural and Neuroendocrinial Assessmment before and after mGluR1 Antagonist (L-AP3) in sheep

Bogdan F. Kania1*, Danuta Wrońska2, Mikołaj A. Gralak3

1. University Veterinary Medicine JU-AU Center, Faculty of Animal Sciences and Biology, Department of Biotechnology, Hugon Kollataj University of Agriculture in, Cracow, Poland

2. Department of Animal Physiology and Endocrinology, Faculty of Animal Sciences and Biology, Hugon Kollataj University of Agriculture in Krakow, Cracow, Poland

3. Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.


The present study examined contribution of mGluRs to the development and maintenance of changes in behavioural and clinical symptoms, blood concentrations of cortisol and catecholamines during visceral pain produced by descsending colonal distension (CD). Experiments were carried out in fourth stages (each of six sheep). Every experiment was performed simultaneously on two unfed animals, which were placed in two individual metabolic cages at one week intervals. Blood was collected 30 min prior to the experiment, at 0 time, and 5, 10, 15, 30, 60 and 120 min after intracerebroventricular (icv) administration of the mGluR1 antagonist: L-2-Amino-3-phosphonopropionic acid (L-AP3, 0.2, 0.4 and/or 0.8 mg in toto), 10 min before, blocked the development of visceral pain symptoms and neuroendocrine changes in the plasma of sheep. This data demonstrated that the development and maintenance of the visceral pain symptoms of CD is dependent on activation of mGluR1 in central nervous system (CNS) and that these receptors play a crucial role in modulating acute jejunal (colic) experimental pain It can be concluded that the mGluR1 antagonists prevent behavioural, clinical and neuroendocrine symptoms of visceral pain. They can be possibly used in cases of acute visceral pain, especially, in combination with opioid agonists. Their simultaneous administration allows to minimise dose of opioids and slows down dynamics of the development of tolerance. This knowledge can be also useful in paliative medicine.

Keywords: L-AP3 (L-2-amino-3-phosphonopropionic acid), Descending Colonal Distension, Catecholamines, Cortisol, Sheep

[PDF]  Viewed: 678  Downloaded: 85
  • Call for paper
    Call for paper

    American Journal of Pharmacy and Health Research (AJPHR) is a peer-reviewed, monthly official international journal allowing access to abstracts and full-text.

  • Email & SMS Alerts
    Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics
    Article Statastics

    American Journal of Pharmacy and Health Research (AJPHR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission
    Online Submission

    American Journal of Pharmacy and Health Research (AJPHR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter